Resumen
Natural killer (NK) cell-based cancer therapies have substantially reshaped modern strategies for clinical cancer treatment and have improved outcomes for countless patients, notably in hematologic malignancies. Unfortunately, its efficacy is limited against solid tumors due to poor infiltration and persistence in the tumor microenvironment. In this study, we show that peripheral blood mononuclear cell-derived NK cell cytotoxicity and persistence can be greatly enhanced to provide highly potent and durable anti-tumor activity through expansion in a feeder cell free-expansion system. Importantly, we show expanded-NK efficacy against three distinct forms of ovarian cancer in mouse models (i.e., solid tumors, abdominal metastatic tumors, and ascites).