Inicio  /  Agriculture  /  Vol: 14 Par: 2 (2024)  /  Artículo
ARTÍCULO
TITULO

Dissecting Diagnostic and Management Strategies for Plant Viral Diseases: What Next?

B. Megala Devi    
Samyuktha Guruprasath    
Pooraniammal Balu    
Anirudha Chattopadhyay    
Siva Sudha Thilagar    
Kanaga Vijayan Dhanabalan    
Manoj Choudhary    
Swarnalatha Moparthi and A. Abdul Kader Jailani    

Resumen

Recent advancements in molecular biology have revolutionized plant disease diagnosis and management. This review focuses on disease diagnosis through serological techniques, isothermal amplification methods, CRISPR-based approaches, and management strategies using RNA-based methods. Exploring high-throughput sequencing and RNA interference (RNAi) technologies like host-induced gene silencing (HIGS) and spray-induced gene silencing (SIGS), this review delves into their potential. Despite the precision offered by RNAi in pest and pathogen management, challenges such as off-target effects and efficient dsRNA delivery persist. This review discusses the significance of these strategies in preventing aphid-mediated plant virus transmission, emphasizing the crucial role of meticulous dsRNA design for effective viral RNA targeting while minimizing harm to plant RNA. Despite acknowledged challenges, including off-target effects and delivery issues, this review underscores the transformative potential of RNA-based strategies in agriculture. Envisaging reduced pesticide dependency and enhanced productivity, these strategies stand as key players in the future of sustainable agriculture.

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