ARTÍCULO
TITULO

Analysis of the 3' end regions of the GDF9 and BMPR1B genes in Blackbelly sheep from Yucatán, Mexico

Rosa B. López-Ramírez    
Héctor F. Magaña-Sevilla    
Roberto Zamora-Bustillos    
Julio P. Ramón-Ugalde    
Daniel González-Mendoza    

Resumen

The FecB mutation in the BMPR1B gene and the G6, G7 and G8 mutations in the GDF9 gene have been documented in Booroola Merino sheep breeds. These mutations have all been identified in several ovine breeds, such as Belclare, Cambridge. Blackbelly hairy sheep breed, which is well adapted to tropical climates due to its African origin, have 2 to 3 lambs per litter, corresponding to a high prolificacy for a tropical breed. The objective of this investigation was to identify mutations in the cDNA of the BMPR1B and GDF9 genes of 20 Blackbelly ewes selected for their prolificacy (two or more lambs by birth). The results of this study indicate the absence of mutations in these genes from the Blackbelly breed. However, it is important to analyze the 3' end region of the cDNA of these genes to rule out the presence or absence of other mutations related to prolificacy.   La mutación FecB del gen BMPR1B es conocido como ?Boorola?, y fue identificada por primera vez en la oveja Merino; las mutaciones G6, G7 y G8 están localizadas en el gen GDF9 y han sido identificadas en varias razas de ovejas, entre ellas, Belclare y Cambridge. La raza de ovinos, conocida como Blackbelly, está bien adaptada a climas tropicales, debido a su origen africano, y el tamaño de su camada al nacimiento es de 2 a 3 corderos. El objetivo de la presente investigación consistió en la identificación de mutaciones en la región 3? del cDNA de los genes GDF9 y BMPR1B, en 20 ovejas multíparas Blackbelly. Los resultados indican la ausencia de estas mutaciones en dicha raza; sin embargo, es importante analizar la región 5` del cDNA de estos genes, para descartar la ausencia o  presencia de otras mutaciones ligadas a la prolificidad.

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