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Chi Zhang, Hanchen Miao, Zhongfang Lei, Tian Yuan, Zhenya Zhang, Ikko Ihara, Hideaki Maseda and Kazuya Shimizu
Antibiotic resistance genes can be spread via gene horizontal transfer (GHT). Chlorination and UV irradiation are common disinfection methods used in wastewater treatment plants before the discharge of treated wastewater. This study aimed to elucidate th...
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Nengwu Si, Jun Li, Hongfei Gao, Yunjing Li, Shanshan Zhai, Fang Xiao, Li Zhang, Gang Wu and Yuhua Wu
In polymerase chain reaction (PCR)-based nucleic acid quantification, the DNA template type, primer/probe sequence, and instrument platform such as real-time quantitative PCR (qPCR) and digital PCR (dPCR) affect the accuracy and reliability of quantitati...
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Ruta Palep?iene, Martynas Maciulevicius, Paulius Ruzgys, Baltramiejus Jak?tys and Saulius ?atkauskas
Gene electrotransfer (GET) is recognized as a promising technique for the development of an efficient tool for gene therapy. Such a therapy would have applications in the treatment of a variety of genetic diseases, including cancer. However, despite its ...
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Frank Wien, Krzysztof Kubiak, Florian Turbant, Kevin Mosca, Grzegorz Wegrzyn and Véronique Arluison
Our results indicate previously unknown macromolecular interplays in the regulation of ColE1-like plasmid DNA replication, which should be important in further studies on combating bacterial antibiotic resistance through controlling the propagation and s...
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Marija Ðordevic, Verica Paunovic, Maja Jovanovic Tucovic, Anja Tolic, Jovana Rajic, Svetlana Dinic, Aleksandra Uskokovic, Nevena Grdovic, Mirjana Mihailovic, Ivanka Markovic, Jelena Aramba?ic Jovanovic and Melita Vidakovic
An efficient transfection is a crucial step for the introduction of epigenetic modification in host cells, and there is a need for an optimized transfection process for individual model systems separately. Mouse pancreatic aTC1-6 cells, which act as an a...
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Ziteng Liu, Junqin Yao, Huiying Ma, Abudukelimu Rukeya, Zenghui Liang, Wenyan Du and Yinguang Chen
Antibiotic resistance genes (ARGs) pose a widespread concern for human health and wastewater treatment plants (WWTPs) are considered to be a major source of ARG transmission. In this paper, the potential hosts and genetic characteristics of ARGs in the i...
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Sonam Chopra, Paulius Ruzgys, Martynas Maciulevicius, Milda Jakutaviciute and Saulius ?atkauskas
Electroporation is an effective method for delivering plasmid DNA molecules into cells. The efficiency of gene electrotransfer depends on several factors. To achieve high transfection efficiency while maintaining cell viability is a tedious task in elect...
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Sung Oh, Chang Won Choi
Pág. 12 - 20
To investigate the pathogenicity and virulence of the Honeysuckle yellow vein virus (HYVV) lacking betasatellites, PCR amplified unit-lengths of DNA-A genome of HYVV-[DJ] were cloned into binary vector pRI101-AN, and generated HYVV-[DJ]-1mer, -1.3mer and...
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Srwa A. Mohammed, Mohammed I. J. Al-ani, Mohammed Dereh L., Lina R. Salar, Banaz M . Rasul
Pág. 1 - 8
Introduction: Enterotoxigenic Staphylococcus aureus is an important pathogen that causes septicemia and bacteremia and is often associated with serious complications, such as endocarditis and osteomyelitis. Some Staphylococcus enterotoxins require only m...
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Ummi Mohlisi Mohd Asmawi,Mohammad Nazmul Hasan Maziz,Mohammad Abdur Rashid,Jamal Houssaini
Pág. 146 - 151
Consumption of undercooked ground beef is the most common route of transmission of verotoxin-producing E.coli. It is estimated that non-O157 verotoxigenic E.coli (VTEC) can cause diarrhea.The sample was isolated from Universiti Malaya Medical Centre. All...
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