Resumen
The studies upstream of the petroleum industry include oil and gas geological exploration and are usually focused on geological, structural, geophysical, and modeling techniques. In this research, the application of a coupled microbiological?isotopic approach was explored to assess its potential as an adequate characterization and monitoring tool of geofluids in oilfield areas, in order to expand and refine the information acquired through more consolidated practices. The test site was selected within the Val d?Agri oilfield, where some natural hydrocarbon springs have been documented since the 19th century in the Tramutola area. Close to these springs, several tens of exploration and production wells were drilled in the first half of the 20th century. The results demonstrated the effectiveness of the proposed approach for the analysis of fluid dynamics in complex systems, such as oilfield areas, and highlighted the capacity of microbial communities to ?behave? as ?bio-thermometers?, that is, as indicators of the different temperatures in various subsurface compartments.