Quantitative Phase Dynamics of Cancer Cell Populations Affected by Blue Light

Resumen

Quantitative phase imaging (QPI) and holographic microscopy (HM) are suitable methods for long-term and label-free imaging of cells and for the complex evaluation of cellular morphological and dynamical parameters under different treatments. Our results suggest that HM within advanced image analysis is capable of correctly distinguishing between subtle changes in cell motility, cell dry mass, cell density, and cell death induced by blue light. As quantitative phase dynamics of the cell population is typical for specific processes in cells, it may be useful for viability tests and medical drugs evaluation. In particular, mapping of the quantitative phase changes during the time seems to be very promising in the determination of various morphological phenomena related to cell death, because the responsible cell death molecular machinery typically changes the distribution of the cell mass.