<b>Effects of polymer pH and molar mass on the PEG/phosphate aqueous two-phase system</b> - doi: 10.4025/actascitechnol.v33i1.7452
Keywords:
poly(ethylene glycol), aqueous two-phase system
Abstract
Liquid-liquid extraction using aqueous two-phase systems (ATPSs) is a promising technique for the purification of biomolecules of industrial and laboratory interest. This work presents a study of the phase diagrams for different poly(ethylene glycol) (PEG) molar masses (ranging from 400 to 4000) and potassium phosphate at pH varying from 6 to 11 in standard conditions. The influence of PEG molecular mass at different pHs was studied from the phase diagrams formed. Stock solutions of PEG 400 and 4000 (100 and 50% w w-1, respectively) and phosphate (20% w w -1 KH2PO4/K2HPO4) were prepared at the appropriate pH (6, 7, 8 and 9). For pHs 10 and 11, NaOH 1M was used in addition to the salts. The results showed that low concentrations were used to form the ATPS with high molecular mass PEG. On the other hand, there was no pH influence in the equilibrium curves – in other words, a significant shift of the binodal in different pHs.Downloads
Download data is not yet available.
Published
2011-02-04
How to Cite
Padilha, G. da S., Ferreira, J. F., Alegre, R. M., & Tambourgi, E. B. (2011). <b>Effects of polymer pH and molar mass on the PEG/phosphate aqueous two-phase system</b> - doi: 10.4025/actascitechnol.v33i1.7452. Acta Scientiarum. Technology, 33(1), 1-4. https://doi.org/10.4025/actascitechnol.v33i1.7452
Issue
Section
Forestry Engineering
DECLARATION OF ORIGINALITY AND COPYRIGHTS
I Declare that current article is original and has not been submitted for publication, in part or in whole, to any other national or international journal.
The copyrights belong exclusively to the authors. Published content is licensed under Creative Commons Attribution 3.0 (CC BY 3.0) guidelines, which allows sharing (copy and distribution of the material in any medium or format) and adaptation (remix, transform, and build upon the material) for any purpose, even commercially, under the terms of attribution.
Read this link for further information on how to use CC BY 3.0 properly.
0.8
2019CiteScore
36th percentile
Powered by
![](/ojs/public/site/images/admin/tecnologia1.png)
![](/ojs/public/site/images/admin/tecnologia1.png)
0.8
2019CiteScore
36th percentile
Powered by ![Scopus](https://www-scopus.ez79.periodicos.capes.gov.br/static/images/scopusLogoOrange.svg)