Técnicas moleculares aplicadas à microbiologia de alimentos - DOI: 10.4025/actascitechnol.v30i1.3245

Eliezer Ávila Gandra; Tatiane Kuka Valente Gandra; Willians Sebastião de Mello; Huana da Silva Godoi

doi:10.4025/actascitechnol.v30i1.3245

Molecular techniques applied to food microbiology: a review - DOI: 10.4025/actascitechnol.v30i1.3245

Eliezer Ávila Gandra Universidade Estadual de Maringá
Tatiane Kuka Valente Gandra UEM
Willians Sebastião de Mello UEM
Huana da Silva Godoi UEM

DOI: https://doi.org/10.4025/actascitechnol.v30i1.3245

Keywords: REA, PFGE, ribotyping, RAPD, RT-PCR, multiplex PCR

Abstract

Beginning in the 1980s, molecular techniques became an alternative to the traditionally used phenotypic methods in food microbiology. With the advent of the polymerase chain reaction technique, this substitution was speed up. This article had as objective to review the main molecular techniques used as tools in food microbiology, from plasmidial profile analysis to contemporary techniques such as the real-time PCR. The characteristics, advantages and disadvantages of these techniques are discussed, by evaluating the potential of these techniques to overcome the limitations of traditional techniques.

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Author Biography

Eliezer Ávila Gandra, Universidade Estadual de Maringá

Possui graduação em Engenharia de Alimentos pela Fundação Universidade Federal do Rio Grande (2001), mestrado em Ciência e Tecnologia Agroindustrial pela Universidade Federal de Pelotas (2003) e doutorado em Ciência e Tecnologia Agroindustrial pela Universidade Federal de Pelotas (2006). Atualmente é professor adjunto da Universidade Estadual de Maringá. Tem experiência na área de Ciência e Tecnologia de Alimentos, com ênfase em Microbiologia de Alimentos, atuando principalmente nos seguintes temas: microrganismos patogênicos, higiene industrial, métodos moleculares aplicados a microbiologia de alimentos. http://lattes.cnpq.br/4340389450218214

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Published

2008-05-08

How to Cite

Gandra, E. Ávila, Gandra, T. K. V., Mello, W. S. de, & Godoi, H. da S. (2008). Molecular techniques applied to food microbiology: a review - DOI: 10.4025/actascitechnol.v30i1.3245. Acta Scientiarum. Technology, 30(1), 109-118. https://doi.org/10.4025/actascitechnol.v30i1.3245

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Issue

Vol 30 No 1 (2008)

Section

Food Technology

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