<b>Evaluation and comparison of the efficiency of detention in chitosan pancreatic lipase for production of fatty acids in flasks under shaking</b> - DOI: 10.4025/actascitechnol.v32i1.7546

Abstract

The enzymatic hydrolysis of oils and fats (or lipolysis) is a technological process that allows the attainment of fatty acids with high aggregate value and low energy consumption. Lipases are enzymes of vegetal, animal or microbial origin that catalyze total or partial hydrolysis of oils and fats. This work had as objective to verify the action of commercial lipase in the hydrolysis reaction of the sunflower oil and corn oil, at time of 24h. Analyses of the main parameters are evaluated (pH, time, temperature and concentration of the substrate), in order to express the maximum of its catalytic activities. The ideal time for better expression of activity in the two substrates was 5 min. It was found that the optimum pH for both substrates was 7.5. The optimum temperature was 50°C in sunflower oil and 40°C in corn oil. The effect of substrate concentration on the activity was at a concentration of 50% for sunflower oil and 30% for corn oil. The best production of total fatty acids was 36.52 g L^-1 using sunflower oil and 29.05 g L^-1 with the corn oil, over a period of 15h of reaction.