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Inicio  /  Acta Scientiarum: Technology  /  Vol: 41 Par: 0 (2019)  /  Artículo
ARTÍCULO
TITULO

Screening of fungal strains with potentiality to hydrolyze microalgal biomass by Fourier Transform Infrared Spectroscopy (FTIR)

Maiara Priscilla de Souza    
Michele Hoeltz    
Maria Viviane Gomes Muller    
Pablo Diego Gressler    
Thiago Rodrigues Bjerk    
Rosana de Cássia de Souza Schneider    
Valeriano Antônio Corbellini    

Resumen

The use of fungi is a promising alternative for harnessing biomass after lipid extraction of microalgae since this biomass may contain relevant levels of carbohydrates that can be converted into other compounds of interest, such as sugars. Fourier Transform Infrared Spectroscopy (FTIR) has proved to be an efficient and environmentally less impacting tool for the selection of microorganisms with biotechnological potential. This study aimed to apply FTIR for the selection of fungal strains with potential to hydrolyze the biomass of the microalgae Desmodesmus subspicatus and Chlorella sp after lipid extraction. Eleven fungal strains were screened for residual biomass hydrolysis and FTIR was applied followed by multivariate analysis for the selection of filamentous fungi. The highest cell density was 28.7 × 106 cells mL-1 for Chlorella sp. and 15.8 × 106 cells mL-1 for D. subspicatus and the values of total carbohydrates content were 23.1 and 16.9%, respectively. Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA) were useful tools to screen fungal strains. After multivariate analysis, it was possible to observe that the fungi strains that presented the greatest ability to use microalgal biomass were Penicillium G12 due to the glucose and xylose sugars obtained after lipid extraction from D. subspicatus (with sugar yield of 9.4 and 6.6%, respectively) and Trichoderma auricularis for Chlorella sp. (with sugar yield of 12.9 and 9.6%, respectively). FTIR was successfully applied to screen fungal strains.

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