Resumen
The world?s arable land has been contaminated by heavy metals to a large extent, which has led to the decreasing availability of farmland. Thus, the remediation of heavy metal pollution deserves due attention, and phytoremediation is preferred. Hemp has been proposed as an ideal alternative crop for remediating heavy-metal-contaminated soil, owing to its well-developed roots, large biomass, and tolerance to heavy metals. Chelators can activate heavy metals to enhance plant absorption, but they may cause stress to plant growth. Therefore, it is very important to optimize the combination of chelator and plant (cultivar) for efficacious phytoremediation. The effects of different concentrations (2, 5, 10, 15, and 25 mmol·L-1) of the chelators disodium ethylene diamine tetra-acetate (EDTA) and citric acid (CA) on the seed germination and plant growth of hemp cultivars were investigated. Triple application of increasing concentrations of EDTA or CA two days apart gradually reduced the germination potential, germination rate, radicle length, and embryonic shoot length of hemp seed, although 2 mmol·L-1 of CA could even promote seed germination. Distinct varietal differences were found in the response of hemp to chelator stress. Under the scheme of four-time uses one week apart, both chelators caused a concentration-dependent linear decrease in the plant height, stem diameter, and biomass of hemp plants, but the growth inhibition due to CA was relatively milder. This could be partially explained by the change in tested physiological indices in hemp leaf. In conclusion, 2 mmol·L-1 of CA helped with seed germination and was almost nontoxic to plant growth; cultivar BM was more tolerant to the chelators than cultivar Y1.