Inicio  /  Applied Sciences  /  Vol: 12 Par: 5 (2022)  /  Artículo
ARTÍCULO
TITULO

Synchrotron Radiation Circular Dichroism, a New Tool to Probe Interactions between Nucleic Acids Involved in the Control of ColE1-Type Plasmid Replication

Frank Wien    
Krzysztof Kubiak    
Florian Turbant    
Kevin Mosca    
Grzegorz Wegrzyn and Véronique Arluison    

Resumen

Our results indicate previously unknown macromolecular interplays in the regulation of ColE1-like plasmid DNA replication, which should be important in further studies on combating bacterial antibiotic resistance through controlling the propagation and spreading of plasmids as carriers of antibiotic resistance genes. New insights on this system are brought by synchrotron radiation-based circular dichroism (SRCD), which gives access to an extended spectral range down to the vacuum UV (vUV) range. Only the synchrotron light source provides intensities of the beam below 190 nm, which allows a precise determination of the nucleic acid conformation.

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