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Nengwu Si, Jun Li, Hongfei Gao, Yunjing Li, Shanshan Zhai, Fang Xiao, Li Zhang, Gang Wu and Yuhua Wu
In polymerase chain reaction (PCR)-based nucleic acid quantification, the DNA template type, primer/probe sequence, and instrument platform such as real-time quantitative PCR (qPCR) and digital PCR (dPCR) affect the accuracy and reliability of quantitati...
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Chi Zhang, Hanchen Miao, Zhongfang Lei, Tian Yuan, Zhenya Zhang, Ikko Ihara, Hideaki Maseda and Kazuya Shimizu
Antibiotic resistance genes can be spread via gene horizontal transfer (GHT). Chlorination and UV irradiation are common disinfection methods used in wastewater treatment plants before the discharge of treated wastewater. This study aimed to elucidate th...
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Ruta Palep?iene, Martynas Maciulevicius, Paulius Ruzgys, Baltramiejus Jak?tys and Saulius ?atkauskas
Gene electrotransfer (GET) is recognized as a promising technique for the development of an efficient tool for gene therapy. Such a therapy would have applications in the treatment of a variety of genetic diseases, including cancer. However, despite its ...
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Frank Wien, Krzysztof Kubiak, Florian Turbant, Kevin Mosca, Grzegorz Wegrzyn and Véronique Arluison
Our results indicate previously unknown macromolecular interplays in the regulation of ColE1-like plasmid DNA replication, which should be important in further studies on combating bacterial antibiotic resistance through controlling the propagation and s...
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Sonam Chopra, Paulius Ruzgys, Martynas Maciulevicius, Milda Jakutaviciute and Saulius ?atkauskas
Electroporation is an effective method for delivering plasmid DNA molecules into cells. The efficiency of gene electrotransfer depends on several factors. To achieve high transfection efficiency while maintaining cell viability is a tedious task in elect...
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Sung Oh, Chang Won Choi
Pág. 12 - 20
To investigate the pathogenicity and virulence of the Honeysuckle yellow vein virus (HYVV) lacking betasatellites, PCR amplified unit-lengths of DNA-A genome of HYVV-[DJ] were cloned into binary vector pRI101-AN, and generated HYVV-[DJ]-1mer, -1.3mer and...
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H. Urzúa, J. M. Urzúa, R. Pizarro
Pág. 3 - 6
The growing enphasis in sustainable agriculture nitrogen fertilization using organic materials motivates the study of improved nitrogen availability in vetch (Vicia benghalensis) grown in alluvial soils of central Chile. With the aim of providing alterna...
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